A Diagnostic Modality Supporting Anatomic Pathology
Immunofluorescence staining is a technique used in the laboratory to diagnose diseases of the skin, kidney, heart, cilia and other organ systems.
Immunofluorescence utilizes a specific pairing between antigen and antibody to identify the deposition of abnormal molecules and proteins in the tissue or cell culture sample. Immunofluorescence (IF) staining labels a specific target antigen with a fluorescent dye such as fluorescein isothiocyanate or cyanine. The fluorescent stain visualized under a fluorescent microscope allows for an assessment of the target molecule/protein distribution in the sample.
Stanford offers both direct immunofluorescence and indirect immunofluorescence, performed by highly qualified staff.
Tissue collection for immunofluorescence requires special handling and transport solutions (Zeus/Michel’s Transport Medium). Please contact the lab to request biopsy kits:
Immunofluorescence/Electron Microscopy Lab
Monday-Friday 8 a.m. to 5 p.m.
Related Collection Guides:
Type IV Collagen: a2, a3 & a5 subchains
IgG subtypes: IgG1, IgG2, IgG3 & IgG4
STANDARD IF PANEL:
IgG, IgM, IgA, C3, C1q, fibrinogen, albumin, kappa and lambda
Alport Diagnosis Panel: Type IV collagen a2, a3 and a5 subchains
Amyloid Panel: Amyloid A, Amyloid P, prealbumin/transthyretin, kappa, lambda
IgG Subtypes: IgG1-4 for MGRS
Membranous Nephropathy: PLA2R (Membranous Nephropathy)
Antigen Retrieval IF on Paraffin Embedded Tissue: For inadequate frozen tissue or MGRS
EPIDERMOLYSIS BULLOSA PANELS:
Primary panel: Antibodies against collagen XVII (BP180), collagen IV, collagen VII, laminin-332 gamma 2 chain
Secondary panel: Antibodies against laminin-332 beta 3 chain, laminin-332 alpha 3 chain, and beta 4 integrin
Antibodies against keratin 5, keratin 14, and plectin are run on an as-needed basis.
DIF Panel: C3, IgG, IgA, IgM, fibrinogen